Transport bosses are being urged to ensure more space for bikes on the new Borders Railway.
Trains on the 30-mile route from Edinburgh to Tweedbank, near Galashiels, will have storage for three cycles per carriage, according to Scottish Government agency Transport Scotland.
But campaigners and business leaders say the line will face heavy demand from cyclists when it opens in 2015.
The Borders regularly hosts stages of the Tour of Britain and the annual ten-day Tweedlove cycling festival. Glentress mountain biking centre, near Peebles, attracts more than 300,000 visitors a year.
Graham Bell, of the Federation of Small Businesses, said: “Three spaces per carriage wouldn’t even allow a family with two children to travel.”