The Playhouse announced this update in a Twitter post on Wednesday and said that protocols to protect their guests and staff against the virus will also remain in place.
A production of White Christmas is currently on at the Edinburgh until December 30.
An Edinburgh Playhouse spokesperson said: "We are proud to be part of the group of theatres that developed and, on re-opening, implemented protocols to keep staff and audiences safe.
"These requirements remain in place.
"They include COVID checks upon entry in the form of either a double vaccination or negative lateral flow test taken within 48 hours of arrival via the NHS self report tool and mandatory mask wearing unless medically exempt.”
The news comes as the UK has recorded the highest daily total of coronavirus cases since the pandemic began, with 78,610 new cases recorded as of 9am on Wednesday.