She says she is recovering but finding the restrictions on her election activities frustrating.
"I came off my bike at the bottom of the Pentlands going round a reservoir," she said. "I've cycled all my life and this is my first accident but it had to be in the middle of an election campaign.”
"It's going to take a bit of time to heal. I'm typing with one hand. But I'm determined to get out and secure each and every vote I can."
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Ms Johnstone, an MSP since 2011 is the Greens' number one candidate on the Lothian list and is also standing in Edinburgh Central, one of Scotland’s most closely-fought seats.
Former Scottish Tory leader Ruth Davidson won it last time with a majority of 610 over the SNP but she is not standing again.